UV spectroscopy can be a powerful tool in the analysis of various biological samples and bodily fluids. The technique has been shown to produce and measure the concentration of iron–sulfur clusters or hemes in a sample by measuring a reduced and oxidized condition of the sample.[x] The technique has also been used to develop a unique label-free method for the detection of human performance “stress” biomarkers in various body fluids.[xi]
UV absorption spectra of amines as a function of concentration in HCl solutions: (a) dopamine; (b) norepinephrine; (c) epinephrine; (d) serotonin.[x]
[x] Gregory P. Holmes-Hampton, … Tracey A. Rouault, “Mitochondrial Function”, Methods in Enzymology, 2014
[xi] Prajokta Ray and Andrew J. Steckl, “Label-Free Optical Detection of Multiple Biomarkers in Sweat, Plasma, Urine, and Saliva”, ACS Sens. 2019, 4, 1346−1357